Omega-3 Plus Ratios Report #2

A BRAND OF MASDIAG

Omega-3 Plus Ratios Report

Referral: ABC Clinic

Name: Sarah Mitchell

DOB: 01/01/2000

Collection Date: 01/01/2026

Received Date: 05/01/2026

Report Date: 08/01/2026

Sample Type: Dried Blood Spot

Analysis: GC-FID

Measurement Unit: Percentage %

TEST0051

Status Reference: Low Suboptimal Optimal Elevated High

Your Omega-3 Index

5.32%

SUBOPTIMAL

Recommendation: 980 mg/day of EPA+DHA from dietary and supplementation sources

5.32%

2.8%4%8% 12%14%15.4%

Optimal

Reference ranges: 2.8 – 15.4%. Optimal range: 8 – 12%.
Reference ranges encompass 99% of fatty acid levels measured in adult populations.

Understanding Your Omega-3 Index Zones

LOW

Below 4%

Increased health risk. Omega-3 intake through diet and supplementation is strongly recommended.

SUBOPTIMAL

4 – 8%

Moderate risk. Dietary improvements and supplementation recommended.

OPTIMAL

8 – 12%

Associated with improved cardiovascular, brain, eye, and joint health, and reduced inflammation.

ELEVATED

12 – 14%

Above optimal. Review supplementation dose with your healthcare provider.

HIGH

Above 14%

Unusually high. Consider reducing omega-3 supplementation.

Summary

Current Level

5.32% Omega-3 Index

Red blood cell EPA + DHA

Optimal Range

8 – 12% Index

Target for optimal health

Current Status

Suboptimal

Below optimal range

Omega-6:3 Ratio

8.3:1

ELEVATED

Optimal: 3:1 – 5:1

AA:EPA Ratio

14.1:1

ELEVATED

Optimal: 2.5:1 – 11:1

📅 Monitoring & Retesting

Low/Suboptimal: Retest in 3–4 months after starting supplementation.
Optimal: Retest in 6 months to ensure levels remain stable.
Elevated/High: Retest in 3 months after reducing intake.
RBC fatty acid levels reflect average intake over the previous 3–4 months.

📍 Recommended Retest: April – May 2026

A BRAND OF MASDIAG

Omega-3 Plus Ratios Report

Referral: ABC Clinic

Name: Sarah Mitchell

DOB: 01/01/2000

Collection Date: 01/01/2026

Received Date: 05/01/2026

Report Date: 08/01/2026

Sample Type: Dried Blood Spot

Analysis: GC-FID

Measurement Unit: Percentage %

TEST0051

Key Results Overview

Omega-3 Index

SUBOPTIMAL

5.32%

Optimal

2.8% 4% 8% 12% 15.4%

Ref: 2.8 – 15.4%. Optimal: 8 – 12%.

Measures EPA and DHA in red blood cells. Optimal level (8–12%) indicates good cardiovascular and anti-inflammatory protection.

Omega-6:Omega-3 Ratio

ELEVATED

8.3:1

Optimal

1:1 3:1 5:1 9:1 11:1

Ref: 1.9:1 – 14.6:1. Optimal: 3:1 – 5:1.

Balance between pro- and anti-inflammatory fatty acids. Lower ratio (3:1–5:1) indicates reduced inflammation.

AA:EPA Ratio

ELEVATED

14.1:1

Optimal

1:1 2.5:1 11:1 20:1 26:1

Ref: 1.1:1 – 69.1:1. Optimal: 2.5:1 – 11:1.

Arachidonic Acid vs EPA balance. Lower ratio indicates better inflammatory balance.

Trans Fat Index

OPTIMAL

0.35%

Optimal

0% 1% 2% 2.5%

Ref: 0.22 – 1.99%. Optimal: < 1%.

Reflects exposure to industrial trans fats, associated with cardiovascular risk. Lower is better.

A BRAND OF MASDIAG

Omega-3 Plus Ratios Report

Referral: ABC Clinic

Name: Sarah Mitchell

DOB: 01/01/2000

Collection Date: 01/01/2026

Received Date: 05/01/2026

Report Date: 08/01/2026

Sample Type: Dried Blood Spot

Analysis: GC-FID

Measurement Unit: Percentage %

TEST0051

Fatty Acid Profile

OMEGA-3 FATTY ACIDS

Fatty Acid	Result (%)	Reference Range	Status
Total Omega-3	4.88	2.7 – 12.9	NORMAL
Omega-3 Index (EPA+DHA)	5.32	2.8 – 15.4	SUBOPTIMAL
Alpha-Linolenic Acid (ALA) C18:3n-3	0.36	0.07 – 0.70	NORMAL
Eicosapentaenoic Acid (EPA) C20:5n-3	0.65	0.20 – 3.85	NORMAL
Docosapentaenoic Acid (DPA) C22:5n-3	1.01	0.63 – 2.60	NORMAL
Docosahexaenoic Acid (DHA) C22:6n-3	2.87	1.76 – 7.78	NORMAL

OMEGA-6 FATTY ACIDS

Fatty Acid	Result (%)	Reference Range	Status
Total Omega-6	40.59	28.4 – 46.2	NORMAL
Linoleic Acid (LA) C18:2n-6	28.06	18.3 – 33.6	NORMAL
Gamma-Linolenic Acid (GLA) C18:3n-6	0.19	0.02 – 0.34	NORMAL
Eicosadienoic Acid C20:2n-6	0.27	0.12 – 0.42	NORMAL
Dihomo-gamma-linolenic (DGLA) C20:3n-6	1.55	0.77 – 2.72	NORMAL
Arachidonic Acid (AA) C20:4n-6	9.11	6.25 – 14.7	NORMAL
Docosatetraenoic Acid C22:4n-6	1.19	0.40 – 2.48	NORMAL
Docosapentaenoic Acid (DPA n-6) C22:5n-6	0.22	0.07 – 0.77	NORMAL

Omega-3 Composition

4.89%

ALA

EPA

DPA

DHA

Omega-6 Composition

40.59%

LA

GLA

EDA

DGLA

AA

DTA

DPA

A BRAND OF MASDIAG

Omega-3 Plus Ratios Report

Referral: ABC Clinic

Name: Sarah Mitchell

DOB: 01/01/2000

Collection Date: 01/01/2026

Received Date: 05/01/2026

Report Date: 08/01/2026

Sample Type: Dried Blood Spot

Analysis: GC-FID

Measurement Unit: Percentage %

TEST0051

Fatty Acid Profile (continued)

TRANS FATTY ACIDS

Fatty Acid	Result (%)	Reference Range	Status
Total Trans Fats	0.50	0.15 – 1.58	NORMAL
Trans Palmitoleic Acid C16:1t	0.15	0.03 – 0.48	NORMAL
Trans Oleic Acid (Elaidic) C18:1t	0.22	0.06 – 0.70	NORMAL
Trans Linoleic Acid C18:2t	0.13	0.05 – 0.40	NORMAL
Trans Fat Index	0.35	< 1.00 (Optimal)	OPTIMAL

FATTY ACID RATIOS

Ratio	Result	Optimal Range	Status
AA:EPA Ratio	14.1:1	2.5:1 – 11:1	ELEVATED
Omega-6:Omega-3 Ratio	8.3:1	3:1 – 5:1	ELEVATED

Tested Fatty Acid Composition

Omega-3 4.88%

Omega-6 40.59%

Trans 0.5%

A BRAND OF MASDIAG

Omega-3 Plus Ratios Report

Referral: ABC Clinic

Name: Sarah Mitchell

DOB: 01/01/2000

Collection Date: 01/01/2026

Received Date: 05/01/2026

Report Date: 08/01/2026

Sample Type: Dried Blood Spot

Analysis: GC-FID

Measurement Unit: Percentage %

TEST0051

Your Results Journey

Your current results with projected improvement at 3–4 months

Omega-3 Index

Omega-6:Omega-3 Ratio

AA:EPA Ratio

Note on Projections

Projected values are based on published research (Walker et al., 2019) and typical responses to a combination of omega-3 supplementation and dietary improvements at the recommended levels. Individual results may vary depending on baseline levels, dietary adherence, genetics, absorption, and lifestyle factors. These projections are not guaranteed outcomes. We recommend retesting in 3–4 months to track your actual progress and adjust your plan accordingly.

A BRAND OF MASDIAG

Omega-3 Plus Ratios Report

Referral: ABC Clinic

Name: Sarah Mitchell

DOB: 01/01/2000

Collection Date: 01/01/2026

Received Date: 05/01/2026

Report Date: 08/01/2026

Sample Type: Dried Blood Spot

Analysis: GC-FID

Measurement Unit: Percentage %

TEST0051

What We Test & Why It Matters

● Omega-3 Index

The Omega-3 Index measures the combined percentage of EPA (Eicosapentaenoic Acid) and DHA (Docosahexaenoic Acid) in your red blood cell membranes. It is the most well-researched and clinically validated marker of long-term Omega-3 status.

Unlike a simple blood test, this reflects your average Omega-3 intake over the past 120 days (the lifespan of red blood cells). An optimal index of 8–12% is associated with improved brain function and reduced systemic inflammation.

● Omega-6:Omega-3 Ratio

This ratio compares total Omega-6 fatty acids to total Omega-3 fatty acids. While both are essential, the modern Western diet typically provides an excess of Omega-6 (from seed oils and processed foods) relative to Omega-3.

An optimal ratio of 3:1 – 5:1 supports balanced inflammatory responses. Higher ratios are associated with increased chronic inflammation, which can contribute to cardiovascular disease, metabolic disorders, and autoimmune conditions.

● AA:EPA Ratio

The AA:EPA ratio measures the balance between Arachidonic Acid (AA, an Omega-6) and Eicosapentaenoic Acid (EPA, an Omega-3). These two fatty acids compete for the same enzymes and directly influence your body’s inflammatory response.

A lower ratio (optimal: 2.5:1 – 11:1) indicates a more anti-inflammatory state. Elevated ratios suggest a pro-inflammatory profile that may be improved through increased EPA intake and reduced consumption of Omega-6-rich foods.

● Trans Fat Index

The Trans Fat Index measures the total percentage of trans fatty acids in your blood. Trans fats are primarily found in partially hydrogenated oils, fried foods, and some processed baked goods.

An optimal level below 1% is desirable. Higher levels are associated with increased LDL cholesterol, decreased HDL cholesterol, and elevated cardiovascular risk. Even small reductions in trans fat intake can have significant health benefits.

Why Optimal Omega-3 Levels Matter

🫀

Heart Health

Reduces risk of cardiovascular events and supports healthy blood pressure

🧠

Brain Function

Supports cognitive performance, memory, and neuroprotection

👁

Eye Health

DHA is essential for retinal health and visual development

😊

Mood & Wellbeing

Linked to reduced symptoms of depression and anxiety

🦴

Joints & Mobility

Reduces inflammation and supports joint comfort

✨

Skin Health

Supports skin hydration, elasticity, and UV protection

🤰

Pregnancy

Critical for foetal brain and eye development

💪

Muscle & Sport

Aids recovery, reduces exercise-induced inflammation

A BRAND OF MASDIAG

Omega-3 Plus Ratios Report

Referral: ABC Clinic

Name: Sarah Mitchell

DOB: 01/01/2000

Collection Date: 01/01/2026

Received Date: 05/01/2026

Report Date: 08/01/2026

Sample Type: Dried Blood Spot

Analysis: GC-FID

Measurement Unit: Percentage %

TEST0051

Diet & Lifestyle

Alongside supplementation, dietary and lifestyle choices play an important role in supporting your omega-3 levels and overall health. The guidance below is tailored to different dietary preferences.

🐟

If You Eat Fish & Seafood

RICHEST EPA+DHA SOURCES

Aim for oily fish 2–3 times per week (salmon, mackerel, sardines, herring, anchovies) — these are the richest natural sources of preformed EPA and DHA.
Shellfish such as mussels, oysters, and shrimp also provide meaningful amounts of omega-3.

EPA & DHA Content Guide

Fish & Seafood (per 100g serving, cooked)

Species	EPA (mg)	DHA (mg)	Total EPA+DHA (mg)	Content
Atlantic Mackerel	504	699	1,203	High
Atlantic Salmon (farmed)	690	1,457	2,147	High
Atlantic Salmon (wild)	411	1,429	1,840	High
Sardines (canned)	473	509	982	High
Herring	909	1,105	2,014	High
Anchovies	763	1,292	2,055	High
Rainbow Trout	334	820	1,154	High
Tuna (canned, light)	44	223	267	Moderate
Cod	4	120	124	Low
Shrimp	50	144	194	Moderate
Mussels	276	506	782	High
Oysters	274	210	484	Moderate

* Values approximate, vary by species/preparation. Source: USDA FoodData Central. High (>500 mg) Moderate (200–500 mg) Low (<200 mg)

🌱

If You Don’t Eat Fish or Are Vegan

PLANT-BASED OPTIONS

Include ALA-rich foods daily: flaxseeds, chia seeds, hemp seeds, and walnuts. Note that ALA conversion to EPA/DHA is limited (typically <5%), so supplementation remains important.
Algae-based omega-3 supplements are an effective vegan source of preformed DHA and EPA, derived directly from marine microalgae rather than fish.

A BRAND OF MASDIAG

Omega-3 Plus Ratios Report

Referral: ABC Clinic

Name: Sarah Mitchell

DOB: 01/01/2000

Collection Date: 01/01/2026

Received Date: 05/01/2026

Report Date: 08/01/2026

Sample Type: Dried Blood Spot

Analysis: GC-FID

Measurement Unit: Percentage %

TEST0051

Lifestyle & Supplementation

🏃

Lifestyle

SUPPORTING FACTORS

Exercise regularly – moderate physical activity supports Omega-3 incorporation into cell membranes and may enhance the anti-inflammatory benefits.
Manage stress – chronic stress increases inflammatory markers and can counteract the benefits of optimal Omega-3 levels.
Body composition – higher body fat percentage is associated with lower Omega-3 levels, as fatty acids distribute across more adipose tissue. Maintaining a healthy weight can support better Omega-3 status.

💊

Supplementation

OMEGA-3 SUPPLEMENTS

Supplement Type	Typical EPA (mg)	Typical DHA (mg)	Total EPA+DHA (mg)	Notes
Standard Fish Oil	180	120	300	Most common, lower concentration
Concentrated Fish Oil	400	200	600	Higher EPA:DHA ratio
Triglyceride Form Fish Oil	350	250	600	Better absorption than ethyl ester
Krill Oil	120	70	190	Phospholipid form, includes astaxanthin
Algae Oil (vegan)	150	300	450	Plant-based, higher DHA
Cod Liver Oil	90	120	210	Includes Vit A & D

💡 Absorption tip: Take omega-3 supplements with or immediately after a meal containing fat. Studies show that consuming omega-3 with a fatty meal can increase absorption by up to 3× compared to taking them on an empty stomach, as dietary fat stimulates bile and lipase production needed to emulsify and absorb the fatty acids.

A BRAND OF MASDIAG

Omega-3 Plus Ratios Report

Referral: ABC Clinic

Name: Sarah Mitchell

DOB: 01/01/2000

Collection Date: 01/01/2026

Received Date: 05/01/2026

Report Date: 08/01/2026

Sample Type: Dried Blood Spot

Analysis: GC-FID

Measurement Unit: Percentage %

TEST0051

Your Personalised EPA+DHA Plan

Use the reference table and interactive calculator to estimate your dietary intake and supplement needs

Your Total Daily Requirement

980 mg/day EPA+DHA

Based on Walker et al. 2019

Target: 8.0% Omega-3 Index

EPA+DHA Content Per Serving

Fish / Seafood	~100g	~150g	~200g
Salmon (farmed)	2,330	3,480	4,630
Salmon (wild)	1,730	2,580	3,430
Mackerel	1,790	2,670	3,550
Sardines (canned)	1,410	2,100	2,790
Herring	1,570	2,340	3,110
Anchovies	1,370	2,040	2,710
Trout (farmed)	1,150	1,710	2,270
Tuna (Bluefin)	1,150	1,710	2,270
Barramundi	400	600	800
Prawns / Shrimp	320	480	640
Snapper	320	480	640
Tuna (canned)	220	330	440
Cod / White fish	140	210	280

Values in mg EPA+DHA per serving. Source: USDA/FSANZ.

Quick Supplement Guide

Your Fish Intake	Diet	Suppl.	Caps
No fish	0	980	3–4
Salmon 1×/wk	~497	~483	2
Salmon 2×/wk	~994	0	0
Sardines 2×/wk	~600	~380	1–2
Canned tuna 2×/wk	~94	~886	3

~300 mg EPA+DHA per standard capsule. Serving sizes ~150g.

Projected values based on Walker et al. (2019). Individual results may vary depending on baseline levels, adherence, genetics, absorption, and lifestyle. Not guaranteed outcomes — retest in 3–4 months. EPA+DHA values approximate (USDA/FSANZ). For educational guidance only.

Interactive Calculator

How many times per week do you eat fish?

Weekly from Fish: 0 mg

Daily from Diet: 0 mg/day

Requirement: 980 mg/day

Supplement Need: 980 mg/day

≈ 3-4 capsules/day (~300 mg EPA+DHA each)

Note: This calculator requires JavaScript. Open in a web browser to use it, or refer to the static table on the left.

A BRAND OF MASDIAG

Omega-3 Plus Ratios Report

Referral: ABC Clinic

Name: Sarah Mitchell

DOB: 01/01/2000

Collection Date: 01/01/2026

Received Date: 05/01/2026

Report Date: 08/01/2026

Sample Type: Dried Blood Spot

Analysis: GC-FID

Measurement Unit: Percentage %

TEST0051

Important Information

🔬

About Fatty Acid Testing

METHODOLOGY

This test analyses a dried blood spot (DBS) sample — whole blood containing RBCs, white blood cells, and plasma — using gas chromatography with flame ionisation detection (GC-FID), quantifying 24 fatty acids expressed as a percentage of total identified fatty acids.
The Omega-3 Index (Harris & von Schacky, 2004) measures EPA + DHA as a percentage of total RBC fatty acids. DBS shows strong correlation (R² > 0.96) with the RBC-based Omega-3 Index, making it a validated method for assessing long-term omega-3 status over 3–4 months.
An Omega-3 Index of 8–12% is considered optimal. This correlation may not extend equally to all individual fatty acids — the complete profile is provided for educational and informational purposes.

📚

References

EVIDENCE BASE

Harris, W.S. & von Schacky, C. (2004). The Omega-3 Index: a new risk factor for death from coronary heart disease? Preventive Medicine, 39(1), 212–220.
Walker, R.E., Jackson, K.H., Tintle, N.L., et al. (2019). Predicting the effects of supplemental EPA and DHA on the omega-3 index. The American Journal of Clinical Nutrition, 110(4), 1034–1040.
Simopoulos, A.P. (2002). The importance of the ratio of omega-6/omega-3 essential fatty acids. Biomedicine & Pharmacotherapy, 56(8), 365–379.
Calder, P.C. (2015). Marine omega-3 fatty acids and inflammatory processes: effects, mechanisms and clinical relevance. Biochimica et Biophysica Acta, 1851(4), 469–484.
Stark, K.D., et al. (2016). Global survey of the omega-3 fatty acids, DHA and EPA in the blood stream of healthy adults. Progress in Lipid Research, 63, 132–152.

⚠️

Disclaimer

IMPORTANT

This report is for informational and educational purposes only and does not constitute medical, dietary, or nutritional advice. The dietary and supplementation recommendations provided are based on published research including Walker et al. (2019) and are intended as general guidance only — they are not a substitute for personalised advice from a qualified healthcare professional, registered dietitian, or nutritionist. Always consult your healthcare provider before making changes to your diet, supplementation, or lifestyle, particularly if you are pregnant, breastfeeding, taking medication, or managing a medical condition. Masdiag does not accept liability for actions taken based on this report.

Questions About Your Results?

CONTACT US

If you have any questions about your results, would like help interpreting your report, or need guidance on next steps, our team is here to help. Please don’t hesitate to reach out — we’re happy to assist you on your health journey.

support@masdiag.com

We aim to respond within 1–2 business days.