Inherited Metabolic Disorders PP

Purines & Pyrimidines.

Comprehensive purine and pyrimidine profiling from urine by LC-MS/MS for detection of approximately 30 enzymatic defects.

Quick Reference
Method
LC-MS/MS
Sample Types
Urine
Analytes

17 analytes

Turnaround

3–5 working days

Enquire About This Test

What does this test assess?

This panel enables detection of approximately 30 enzymatic defects in purine and pyrimidine metabolism through quantitative analysis of 17 bases and metabolites.

Clinical indications include:

  • Diagnosis of Lesch-Nyhan syndrome (HPRT deficiency)
  • Detection of adenosine deaminase (ADA) deficiency
  • Identification of dihydropyrimidine dehydrogenase deficiency (5-FU toxicity risk)
  • Investigation of immunodeficiency, neurological symptoms, or unexplained anaemia
  • Screening for orotic aciduria and related disorders

Measured analytes

Analyte coverage

17 purine and pyrimidine bases including uric acid, xanthine, hypoxanthine, adenine, guanine, orotic acid, uracil, thymine, and related metabolites.

Analytical technique

All 17 analytes are quantified simultaneously from a single urine sample by LC-MS/MS, with stable isotope internal standards ensuring accuracy across the concentration range.

Testing process

From enquiry to results — a straightforward process.

1
Get in touch
Contact us to discuss your testing requirements
2
Collect your sample
Collect a urine sample using the provided collection kit
3
Send to our lab
Urine samples are shipped to our laboratory following standard protocols
4
Receive results
Results delivered within 3–5 working days of sample receipt

Where this test is available

This test is available to healthcare professionals, wellness brands, clinics, and research institutions worldwide. We currently serve partners in:

  • Europe (EU & non-EU)
  • United Kingdom
  • Asia & Southeast Asia
  • Australia & New Zealand
  • United States

Whether you need testing services for your patients, white-label kits for your brand, or method transfer to your own laboratory — get in touch to discuss how we can work together.

Frequently Asked Questions

What does this test measure?

This test provides quantitative and qualitative analysis of purines, pyrimidines, and related metabolites in urine by LC-MS/MS. It screens for approximately 30 enzymatic defects in nucleotide metabolism by measuring 17 key bases and metabolites.

What conditions can it detect?

The test can detect Lesch-Nyhan syndrome (HPRT deficiency), adenosine deaminase (ADA) deficiency, purine nucleoside phosphorylase deficiency, dihydropyrimidine dehydrogenase deficiency (which is also important for predicting 5-fluorouracil toxicity risk), orotic aciduria, and other rare but clinically significant disorders of nucleotide metabolism.

How is the sample collected?

A random urine sample is collected — no special timing or fasting is required. The urine is collected using a standard collection container and shipped to our laboratory following standard protocols.

How long does it take to get results?

Results are typically delivered within 3 to 5 working days from the time your sample arrives at our laboratory. The analysis is performed by LC-MS/MS, providing accurate quantification of purine and pyrimidine metabolites.

When should purine and pyrimidine testing be considered?

Testing should be considered in cases of unexplained neurological symptoms (especially in children), severe combined immunodeficiency (SCID), unexplained gout or renal stones at a young age, developmental delay with no other identified cause, or when investigating potential 5-fluorouracil toxicity risk prior to chemotherapy.

Which countries is this test available in?

Masdiag's purines and pyrimidines test is available worldwide through our partner network. We currently serve healthcare professionals, metabolic disease centres, and clinics in Europe, the United Kingdom, Asia, Australia, New Zealand, and the United States. Contact us to discuss testing services or method transfer.

Interested in this method?

Whether you need testing services, method transfer, or white-label kit development — we'd love to hear from you.